Use of trypan blue for penetrating keratoplasty was developed to facilitate the procedure. Trypan blue is injected before and after the addition of 0.25 mL of an ophthalmic viscosurgical device (OVD), sodium hyaluronate, to stain the internal and external cut edge of the cornea as well as the OVD, enabling the surgeon to improve visualization of the incision and suture depth, improve alignment of host and donor tissues, and ensure that all OVD is removed.
From the Department of Medicine (Roos) and the Department of Ophthalmology (Kerr Muir), Addenbrooke's Hospital, University of Cambridge, Cambridge, United Kingdom
Reprint requests to Jonathan C.P. Roos, MA, GIBiol, Department of Medicine, Level 5, Addenbrooke's Hospital, University of Cambridge, CB2 2QQ Cambridge, United Kingdom.
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The medical photography staff at Cambridge University Teaching Hospitals, National Health Service Trust, processed the visual images.
No author has a financial or proprietary interest in any material or method mentioned.
ABSTRACT
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