Journal of Cataract & Refractive Surgery
Volume 32, Issue 11 , Pages 1784-1791 , November 2006

Noninvasive corneal stromal collagen imaging using two-photon-generated second-harmonic signals

,Accepted 7 April 2006.

References 

  1. Hamada R, Giraud J-P, Graf B, Pouliquen Y. Étude analytique et statistique des lamelles, des keratocytes, des fibrilles de collagène de la région centrale de la cornée humaine normale. (Microscopie optique et électronique). Arch Ophtalmol Rev Gen Ophtalmol. 1972;32:563–570
  2. Komai Y, Ushiki T. The three-dimensional organization of collagen fibrils in the human cornea and sclera. Invest Ophthalmol Vis Sci. 1991;32:2244–2258
  3. Radner W, Zehetmayer M, Aufreiter R, Mallinger R. Interlacing and cross-angle distribution of collagen lamellae in the human cornea. Cornea. 1998;17:537–543
  4. Binder PS, Rock ME, Schmidt KC, Anderson JA. High-voltage electron microscopy of normal human cornea. Invest Ophthalmol Vis Sci. 1991;32:2234–2243
  5. Müller LJ, Pels L, Vrensen GFJM. Novel aspects of the ultrastructural organization of human corneal keratocytes. Invest Ophthalmol Vis Sci. 1995;36:2557–2567
  6. Müller LJ, Pels E, Vrensen GFJM. The specific architecture of the anterior stroma accounts for maintenance of corneal curvature. Br J Ophthalmol. 2001;85:437–443
  7. Müller LJ, Pels E, Schurmans LRHM, Vrensen GFJM. A new three-dimensional model of the organization of proteoglycans and collagen fibrils in the human corneal stroma. Exp Eye Res. 2004;78:493–501
  8. Aghamohammadzadeh H, Newton RH, Meek KM. X-Ray scattering used to map the preferred collagen orientation in the human cornea and limbus. Structure. 2004;12:249–256
  9. Boote C, Dennis S, Huang Y, et al. Lamellar orientation in human cornea in relation to mechanical properties. J Struct Biol. 2005;149:1–6
  10. Freund I, Deutsch M, Sprecher A. Connective tissue polarity; optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in rat-tail tendon. Biophys J. 1986;50:693–712
  11. Mohler W, Millard AC, Campagnola PJ. Second harmonic generation imaging of endogenous structural proteins. Methods. 2003;29:97–109
  12. Campagnola PJ, Loew LM. Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms. Nat Biotechnol. 2003;21:1356–1360
  13. Hochheimer BF. Second harmonic light generation in the rabbit cornea. Appl Opt. 1982;21:1516–1518
  14. Williams RM, Zipfel WR, Webb WW. Multiphoton microscopy in biological research. Curr Opin Chem Biol. 2001;5:603–608
  15. Stoller P, Kim B-M, Rubenchik AM, et al. Polarization-dependent optical second-harmonic imaging of a rat-tail tendon. J Biomed Opt. 2002;7:205–214
  16. Yeh AT, Nassif N, Zoumi A, Tromberg BJ. Selective corneal imaging using combined second-harmonic generation and two-photon excited fluorescence. Optics Lett. 2002;27:2082–2084
  17. Zoumi A, Yeh A, Tromberg BJ. Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence. PNAS. 2002;99:11014–11019
  18. Han M, Zickler L, Giese G, et al. Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation. J Biomed Opt. 2004;9:760–766
  19. Goldman JN, Benedek GB. The relationship between morphology and transparency in the nonswelling corneal stroma of the shark. Invest Ophthalmol. 1967;6:574–600
  20. Keller N, Pouliquen Y. Ultrastructural study of the posterior cornea of the dogfish “Scyliorhinus canicula L.” Cornea 1985–86; 4:108–117
  21. Bron AJ. The architecture of the corneal stroma. [editorial] Br J Ophthalmol. 2001;85:379–381
  22. Maurice DM, Monroe F. Cohesive strength of corneal lamellae. Exp Eye Res. 1990;50:59–63
  23. Smolek MK, McCarey BE. Interlamellar adhesive strength in human eyebank corneas. Invest Ophthalmol Vis Sci. 1990;31:1087–1095
  24. Bettelheim FA. The hydration of proteoglycans of bovine cornea. Biochim Biophys Acta. 1975;381:203–214
  25. Twa MD, Nichols JJ, Joslin CE, et al. Characteristics of corneal ectasia after LASIK for myopia. Cornea. 2004;23:447–457
  26. Pallikaris IG, Kymionis GD, Astyrakakis NI. Corneal ectasia induced by laser in situ keratomileusis. J Cataract Refract Surg. 2001;27:1796–1802
  27. Randleman JB, Russell B, Ward MA, et al. Risk factors and prognosis for corneal ectasia after LASIK. Ophthalmology. 2003;110:267–275

 No author has a proprietary or financial interest in any material or method mentioned.Supported in part by National Institutes of Health grants EY07348 (Dr. Jester) and Infrastructure Grant EY016663, Lew Wasserman Merit Award and Support Grant from Research to Prevent Blindness, Inc., The Skirball Program in Molecular Ophthalmology, and the Japan Eye Bank Association.

PII: S0886-3350(06)01121-7

doi: 10.1016/j.jcrs.2006.08.027

Journal of Cataract & Refractive Surgery
Volume 32, Issue 11 , Pages 1784-1791 , November 2006