Objective measurement of intraocular forward light scatter using Hartmann-Shack spot patterns from clinical aberrometers: Model-eye and human-eye study
Accepted 30 March 2008.
Purpose
To apply software-based image-analysis tools to objectively determine intraocular scatter determined from clinically derived Hartmann-Shack patterns.
Setting
Aston Academy of Life Sciences, Aston University, Birmingham, United Kingdom, and Department of Optics, University of Valencia, Valencia, Spain.
Methods
Purpose-designed image-analysis software was used to quantify scatter from centroid patterns obtained using a clinical Hartmann-Shack analyzer (WASCA, Zeiss/Meditec). Three scatter values, as the maximum standard deviation within a lenslet for all lenslets in the pattern, were obtained in 6 model eyes and 10 human eyes. In the model-eye sample, patterns were obtained in 4 sessions: 2 without realigning between measurements, 1 with realignment, and 1 with an angular shift of 6 degrees from the instrument axis. Three measurements were made in the human eyes with the C-Quant straylight meter (Oculus) to obtain psychometric and objective measures of retinal straylight. Analysis of variance, intraclass correlation coefficients, coefficient of repeatability (CoR), and correlations were used to determine intrasession and intersession repeatability and the relationship between measures.
Results
No significant differences were found between the sessions in the model eye (P = .234). The mean CoR was less than 10% in all model- and human-eye sessions. After incomplete patterns were removed, good correlation was achieved between psychometric and objective scatter measurements despite the small sample size (n = 6; r = −0.831; P = .040).
Conclusions
The methodology was repeatable in model and human eyes, strong against realignment and misalignment, and sensitive. Clinical application would benefit from effective use of the sensor's dynamic range.
From the Department of Optics (Cerviño, Montés-Micó), University of Valencia, Valencia, Spain; Optopol Technology SA (Bansal), Zawiercie, Poland; the Department of Optometry and Vision Sciences (Hosking), City University, London, and the School of Life and Health Sciences (Hosking), Aston University, Birmingham, United Kingdom; and the Department of Ophthalmology (Hosking), University of Melbourne, Melbourne, Australia
Corresponding author: Dr. Alejandro Cerviño, Optometry Research Group, Department of Optics, University of Valencia, Dr. Moliner, 50, 46100 Burjassot, Valencia, Spain.
No author has a financial or proprietary interest in any material or method mentioned.
ABSTRACT