Experimental use of estriol for visualizing the vitreous body in the anterior chamber after posterior capsule rupture in animal models
Received 2 January 2009; received in revised form 2 February 2009; accepted 4 February 2009.
Purpose
To compare the efficacy and safety of estriol and triamcinolone acetonide suspensions in visualizing the prolapsed vitreous body in the anterior chamber after posterior capsule rupture in animal models.
Setting
Tsukuba University Institute of Clinical Medicine, Ibaraki, Japan.
Methods
To evaluate efficacy, triamcinolone acetonide or estriol suspension was injected into the anterior chambers of porcine eyes after intentional posterior capsule rupture. To evaluate safety, triamcinolone acetonide 5.0 mg or estriol in 0.1 mL suspension was injected into the anterior chamber of New Zealand white rabbits. Slitlamp examinations, intraocular pressure (IOP), corneal endothelial cell density (ECD) measurements, and histologic examinations were performed up to 28 days after the injection.
Results
Triamcinolone acetonide and estriol were equally effective in allowing visualization of the prolapsed vitreous body in the anterior chamber. The granules of triamcinolone acetonide or estriol disappeared 1 day after the injection and did not affect the IOP or corneal ECD. No statistically significant histological changes were observed in the eyes 28 days after the injection of triamcinolone acetonide or estriol.
Conclusions
Estriol was effective for the visualization of the prolapsed vitreous body in the anterior chamber after posterior capsule rupture. In experimental models, no significant side effects were observed after the injection of estriol in the anterior chamber. Results suggest that estriol is an alternative reagent for visualizing the vitreous body, especially in steroid responders, because it has no glucocorticoid or mineralocorticoid activity.
From the Department of Ophthalmology, Tsukuba University Institute of Clinical Medicine, Ibaraki, Japan
Corresponding author: Yuichi Kaji, MD, PhD, Department of Ophthalmology, Tsukuba University Institute of Clinical Medicine, Tennoudai 1-1-1, Tsukuba Ibaraki 305-8575, Japan.
No author has a financial or proprietary interest in any material or method mentioned.
Supported by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Young Scientists, 18791259 (2006–2008), and for Scientific Research, 21592216 (2009-2011), Japan.
ABSTRACT